Name: Anti-Ctip2 antibody 25B6 - ChIP Gradeab18465
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Anti-Ctip2 antibody [25B6] - ChIP Grade產(chǎn)品信息：

Product name：Anti-Ctip2 antibody [25B6] - ChIP Grade
Description
Rat monoclonal [25B6] to Ctip2 - ChIP Grade
SpecificityDetects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
Tested applicationsICC/IF, IHC-FoFr, WB, IP, IHC-P, IHC-Fr, ChIP, IHC-FrFl, Flow Cyt
Species reactivity
Reacts with: Mouse, Guinea pig, Human, Zebrafish
Immunogen
Fusion protein (Human).
EpitopeBetween amino acids 1-150 of CTIP2.
Positive control
- WB: nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody IHC: adult mouse hippocampus, Adult mouse spinal cord ICC: Neonatal mouse hippocampal cultured neurons
General notesHybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

Properties

FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 150mM Sodium chloride, 15mM HEPES, pH 7.5
Concentration100 µg at 1 mg/ml
PurityIgG fraction
ClonalityMonoclonal
Clone number25B6
IsotypeIgG2a

Application	Notes
ICC/IF	ICC/IF: 1/500.
IHC-FoFr	IHC-FoFr: Use at an assay dependent dilution. PubMed: 18523013
WB	WB: Use at an assay dependent dilution. Detects a band of approximay 120 kDa (predicted molecular weight: 95 kDa).
IP	IP: Use at an assay dependent dilution.
IHC-P	IHC-P: 1/500.
IHC-Fr	IHC-Fr: 1/500.
ChIP	ChIP: Use at an assay dependent dilution.
IHC-FrFl	IHC-FrFl: Use at an assay dependent concentration.
Flow Cyt	Flow Cyt: Use 1µg for 10⁶ cells.

Target

FunctionTumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Tissue specificityHighly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
Sequence similaritiesContains 6 C2H2-type zinc fingers.
Cellular localizationNucleus.
Anti-Ctip2 antibody [25B6] - ChIP Grade images
Immunohistochemistry (Frozen sections) - Ctip2 antibody [25B6] - ChIP Grade (ab18465)This image is courtesy of an anonymous Abreview
ab18465 staining Ctip2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor^®488-conjugated Goat polyclonal to rat IgG (1/1000) was used as secondary antibody. Staining was improved with citrate antigen retrieval.
Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)
Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).
Red is beta tubulin staining.
Green is ab18465.
Immunocytochemistry - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
Immunohistochemistry using ab18465 on Adult Mouse Hippocampus.
Immunocytochemistry/ Immunofluorescence - Ctip2 antibody [25B6] - ChIP Grade (ab18465)
Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.
Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)
Predicted band size : 95 kDa
Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).
Western blot - Ctip2 antibody [25B6] - ChIP Grade (ab18465)
All lanes : Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) at 1/500 dilution

Lane 1 : Mouse brain tissue lysate at 1.5 µg
Lane 2 : Mouse brain tissue lysate at 1.5 µg
Lane 3 : Mouse brain tissue lysate at 3 µg

Secondary
IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 95 kDa
Observed band size : 100,110 kDa

Exposure time : 10 minutes
This image is courtesy of an Anonymous Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465)
ab18465 staining Ctip2 in Guinea Pig Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.
Flow Cytometry-Anti-Ctip2 antibody [25B6] - ChIP Grade(ab18465)
Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
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